中/英文名称：

内收蛋白抗体/ Anti- beta Adducin

别名：

ADD 2; ADD B; ADD2; ADDB; ADDL; Adducin 2 (beta);   Adducin 2; Adducin like; Adducin like protein 70; Adducin2; Beta adducin;   Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha;   Erythrocyte adducin subunit beta; MGC3339; MGC44427; ADDB_HUMAN.

产品编号：

zy10022

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human beta Adducin protein

交叉反应：

Human, Mouse, Rat, Dog, Horse, Rabbit

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 80kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Adducins are a family of cytoskeletal proteins   encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric   protein that consists of related subunits, which are produced from distinct   genes but share a similar structure. Alpha and beta adducin include a   protease-resistant N-terminal region and a protease-sensitive, hydrophilic   C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In   contrast, beta adducin is expressed at high levels in brain and hematopoietic   tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a   substrate for protein kinases A and C. Alternative splicing results in   multiple variants encoding distinct isoforms; however, not all variants have   been fully described.

其他:

Function:

Membrane-cytoskeleton-associated protein that   promotes the assembly of the spectrin-actin network. Binds to calmodulin.   Calmodulin binds preferentially to the beta subunit.

Subunit:

Heterodimer of an alpha and a beta subunit.

Subcellular   Location:

Cytoplasm, cytoskeleton. Cell membrane;   Peripheral membrane protein; Cytoplasmic side.

Tissue   Specificity:

Expressed mainly in brain, spleen, kidney cortex   and medulla, and heart. Also expressed in human umbilical vein endothelial   cells, human vascular smooth muscle cells, kidney tubular cells and K-562   cell line.

Post-translational   modifications:

The N-terminus is blocked.

Similarity:

Belongs to the aldolase class II family.   Adducinbn subfamily.

Gene   ID:

119

内收蛋白(Adducin）是一种膜骨架蛋白，内收蛋白可与肌动蛋白及血影蛋白复合体结合，并且通过Ca2+和钙调蛋白的作用影响骨架蛋白的稳定性，从而影响红细胞的形态.