中/英文名称：

血管紧张素转换酶ACE1抗体/ Anti-ACE

别名：

Angiotensin Converting Enzyme 1; ACE; ACE-T;   Angiotensin-converting enzyme isoform 1precursor; Dipeptidyl carboxy   peptidase 1; Kininase II; ACE-1;testis-specific isoform precursor. ACE 1; ACE   T; ACE1; Angiotensin converting enzyme somatic isoform; Angiotensin   converting enzyme testis specific isoform; Angiotensin I converting enzyme;   Angiotensin I converting enzyme 1; Angiotensin I converting enzyme peptidyl   dipeptidase A 1; Carboxycathepsin; CD 143; CD143; CD143 antigen; DCP 1; DCP;   DCP1; Dipeptidyl carboxypeptidase 1; MVCD3; Peptidase P; Peptidyl dipeptidase   A; Testicular ECA; ACE_HUMAN.

产品编号：

zy10010

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ACE

交叉反应：

Human, Mouse, Rat, Dog, Pig, Cow, Sheep

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 147kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Angiotensin Converting enzyme is involved in   catalyzing the conversion of angiotensin I into a physiologically active   peptide angiotensin II. Angiotensin II is a potent vasopressor and   aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte   balance. This enzyme plays a key role in the renin-angiotensin system. ACE   converts angiotensin I to angiotensin II by release of the terminal His-Leu,   this results in an increase of the vasoconstrictor activity of angiotensin.   Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two   forms, a 170KD somatic form and a 90KD germinal form. The somatic form is   expressed by endothelial cells (especially those of lung capillaries and   arterioles), epithelial cells (especially in proximal renal tubules and small   intestine), by some neuronal cells and variably by some macrophages and T   lymphocytes. The germinal form is expressed by spermatozoa.

其他:

Function:

Converts angiotensin I to angiotensin II by   release of the terminal His-Leu, this results in an increase of the   vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin,   a potent vasodilator. Has also a glycosidase activity which releases   GPI-anchored proteins from the membrane by cleaving the mannose linkage in   the GPI moiety.

Subcellular   Location:

Angiotensin-converting enzyme, soluble form:   Secreted.

Cell membrane; Single-pass type I membrane   protein.

Tissue   Specificity:

Ubiquitously expressed, with highest levels in   lung, kidney, heart, gastrointestinal system and prostate. Isoform   Testis-specific is expressed in spermatocytes and adult testis.

Post-translational   modifications:

Phosphorylated by CK2 on Ser-1299; which allows   membrane retention.

DISEASE:

Genetic variations in ACE may be a cause of   susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as   cerebrovascular accident or cerebral infarction. A stroke is an acute   neurologic event leading to death of neural tissue of the brain and resulting   in loss of motor, sensory and/or cognitive function. Ischemic strokes,   resulting from vascular occlusion, is considered to be a highly complex   disease consisting of a group of heterogeneous disorders with multiple   genetic and environmental risk factors.

Defects in ACE are a cause of renal tubular   dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder   of renal tubular development characterized by persistent fetal anuria and   perinatal death, probably due to pulmonary hypoplasia from early-onset   oligohydramnios (the Potter phenotype).

Genetic variations in ACE are associated with   susceptibility to microvascular complications of diabetes type 3 (MVCD3)   [MIM:612624]. These are pathological conditions that develop in numerous   tissues and organs as a consequence of diabetes mellitus. They include   diabetic retinopathy, diabetic nephropathy leading to end-stage renal   disease, and diabetic neuropathy. Diabetic retinopathy remains the major   cause of new-onset blindness among diabetic adults. It is characterized by   vascular permeability and increased tissue ischemia and angiogenesis.

Similarity:

Belongs to the peptidase M2 family.

Gene   ID:

1636

ACE的主要功能是转化血管紧张素Ⅰ为血管紧张素Ⅱ，后者有升高血压的作用。大多数结节病活动期ACE活性升高.

Tissue/cell: rat pancreas   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate   buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous   peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat   serum) at 37℃ for 20 min;

Incubation: Anti-ACE1   Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by   conjugation to the secondary antibody and DAB staining