中/英文名称：

Acinus抗体/   Anti- Acinus

别名：

ACIN 1; ACIN1; ACINU_HUMAN; Acinus; ACN;   Apoptotic chromatin condensation inducer 1; Apoptotic chromatin condensation   inducer in the nucleus; DKFZp667N107; fSAP152; functional   spliceosome-associated protein 152; KIAA0670.

产品编号：

zy10013

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human Acinus

交叉反应：

Human, Mouse, Rat, Dog, Cow, Horse

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 148kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Chromatin condensation and nuclear fragmentation   (CCNF) are the hallmarks of apoptosis. CCNF is triggered by the activation of   members of the caspase family, caspase activated DNase (CAD/DFF40), and   several novel proteins including AIF and CIDE. A new inducer of chromatin   condensation was recently identified and designated Acinus (for apoptotic   chromatin condensation inducer in the nucleus). Acinus is cleaved by Caspase   3 and an additional unknown protease generating a small active peptide p17,   which causes chromatin condensation in vitro when it is added to purified   nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus   is ubiquitously expressed. Three different spliced forms of Acinus have been   identified in human and mouse and designated Acinus L (1341 amino acids),   Acinus S (583 amino acids) and Acinus S' (614 amino acids)

其他:

Function:

Component of a splicing-dependent multiprotein   exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is   a dynamic structure consisting of a few core proteins and several more   peripheral nuclear and cytoplasmic associated factors that join the complex   only transiently either during EJC assembly or during subsequent mRNA   metabolism. Induces apoptotic chromatin condensation after activation by   CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

Subunit:

Found in a mRNA splicing-dependent exon junction   complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, PNN, RBM8A,   RNPS1, SAP18 and ALYREF/THOC4. Forms heterodimers with RNPS1. Found in a   heterotrimeric complex with ACIN1, RNPS1 and SAP18. Interacts with API5.   Interacts with SRPK2 in a phosphorylation-dependent manner.

Subcellular   Location:

Nucleus. Nucleus speckle. Nucleus, nucleoplasm.   Note=Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear   speckles to the nucleoplasm.

Tissue   Specificity:

Ubiquitous. The Ser-1180 phosphorylated form (by   SRPK2) is highly expressed and phosphorylated in patients with myeloid   hematologic malignancies.

Post-translational   modifications:

Phosphorylation on Ser-1180 by SRPK2 up-regulates   its stimulatory effect on cyclin A1.

Undergoes proteolytic cleavage; the processed   form is active, contrary to the uncleaved form.

Similarity:

Contains 1 SAP domain.

Gene   ID:

22985