中/英文名称：

醋酸激酶1抗体/ Anti-Ack1

别名：

Acetate kinase 1; Acetokinase 1; Activated CDC42   kinase 1; Activated Cdc42 associated kinase 1; Activated p21cdc42Hs kinase;   Tyrosine kinase non receptor 2; Tyrosine kinase non-receptor protein 2;   non-receptor protein tyrosine kinase ACK; ACK; TNK 2; Tnk2; ACK1; FLJ44758;   FLJ45547; ACK 1; ACK-1; ACK1_HUMAN; Activated p21cdc42Hs kinase; FLJ44758;   FLJ45547; Tyrosine kinase non receptor protein 2; p21cdc42Hs.

产品编号：

zy10014

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human Ack1 N-terminus

交叉反应：

Human, Mouse, Rat, Chicken, Dog, Pig, Cow

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 119kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

This gene encodes a tyrosine kinase that binds   Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and   GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This   binding is mediated by a unique sequence of 47 amino acids C-terminal to an   SH3 domain. The protein may be involved in a regulatory mechanism that   sustains the GTP-bound active form of Cdc42Hs and which is directly linked to   a tyrosine phosphorylation signal transduction pathway. Several alternatively   spliced transcript variants have been identified from this gene, but the   full-length nature of only two transcript variants has been determined.   [provided by RefSeq].

其他:

Function:

Non-receptor tyrosine-protein and   serine/threonine-protein kinase that is implicated in cell spreading and   migration, cell survival, cell growth and proliferation. Transduces   extracellular signals to cytosolic and nuclear effectors. Phosphorylates   AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and   clathrin-mediated endocytosis through binding to epidermal growth factor   receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and   regulates ligand-induced degradation of EGFR, thereby contributing to the   accumulation of EGFR at the limiting membrane of early endosomes. Downstream   effector of CDC42 which mediates CDC42-dependent cell migration via   phosphorylation of BCAR1. May be involved both in adult synaptic function and   plasticity and in brain development. Activates AKT1 by phosphorylating it on   'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its   recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on   'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine   nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to   the control of AXL receptor levels. Confers metastatic properties on cancer   cells and promotes tumor growth by negatively regulating tumor suppressor   such as WWOX and positively regulating pro-survival factors such as AKT1 and   AR.

Subunit:

Interacts with NEDD4 (via WW3 domain). NEDD4L and   EGF promote association with NEDD4 (By similarity). Homodimer. Interacts with   AR, CDC42, WWASL and WWOX. Interacts with CSPG4 (activated). Interacts with   MERTK (activated); stimulates autophosphorylation. May interact   (phosphorylated) with HSP90AB1; maintains kinase activity. Interacts with   NPHP1. Interacts with SNX9 (via SH3 domain). Interacts with SRC (via SH2 and   SH3 domain). Interacts with EGFR, and this interaction is dependent on EGF   stimulation and kinase activity of EGFR. Interacts (via kinase domain) with   AKT1. Part of a collagen stimulated complex involved in cell migration   composed of CDC42, CRK, TNK2 and BCAR1/p130cas. Interacts with BCAR1/p130cas   via SH3 domains. Forms complexes with GRB2 and numerous receptor tyrosine   kinases (RTK) including LTK, AXL or PDGFRL, in which GRB2 promotes RTK   recruitment by TNK2.

Subcellular   Location:

Cell membrane. Nucleus. Endosome. Cell junction,   adherens junction. Cytoplasmic vesicle membrane; Peripheral membrane protein;   Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane,   clathrin-coated pit. Note=The Tyr-284 phosphorylated form is found both in   the membrane and nucleus. Co-localizes with EGFR on endosomes. Nuclear   translocation is CDC42-dependent.

Tissue   Specificity:

The Tyr-284 phosphorylated form shows   asignificant increase in expression in breast cancers during theprogressive   stages i.e. normal to hyperplasia (ADH), ductalcarcinoma in situ (DCIS),   invasive ductal carcinoma (IDC) and lymphnode metastatic (LNMM) stages. It   also shows a significant increasein expression in prostate cancers during the   progressive stages.

Post-translational   modifications:

Autophosphorylation regulates kinase activity.   Phosphorylation on Tyr-518 is required for interaction with SRC and is   observed during association with clathrin-coated pits.

Polyubiquitinated by NEDD4 and NEDD4L.   Degradation can be induced by EGF and is lysosome-dependent (By similarity).

Similarity:

Belongs to the protein kinase superfamily. Tyr   protein kinase family.

Contains 1 CRIB domain.

Contains 1 protein kinase domain.

Contains 1 SH3 domain.

Contains 1 UBA domain.

Gene   ID:

10188

Ack 1分子是一组促进酪氨酸激酶生长的家族成员，该蛋白与肿瘤分裂、生长、转移起到很重要的作用，可加速恶性肿瘤的浸润。

Tissue/cell: human colon   carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-Ack1 Polyclonal Antibody, Unconjugated 1:200, overnight at   4°C, followed by conjugation to the secondary antibody and DAB staining