中/英文名称：

促肾上腺皮质激素(18-39)抗体/ Anti- ACTH (18-39)

别名：

Adrenocorticotropic hormone; Adrenocorticotropin;   Adrenocorticotropin Hormone; Alpha Melanocyte Stimulating Hormone; Beta   Lipotropin; Beta Melanocyte Stimulating Hormone; CLIP; Corticotropin;   Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor;   Lipotropin Beta; Lipotropin Gamma; LPH; Melanotropin Alpha; Met Enkephalin;   MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin; COLI_HUMAN.

产品编号：

zy10016

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ACTH(18-39)

交叉反应：

Human

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 2.5/4.5kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

This gene encodes a polypeptide hormone precursor   that undergoes extensive, tissue-specific, post-translational processing via   cleavage by subtilisin-like enzymes known as prohormone convertases. There   are eight potential cleavage sites within the polypeptide precursor and,   depending on tissue type and the available convertases, processing may yield   as many as ten biologically active peptides involved in diverse cellular   functions. The encoded protein is synthesized mainly in corticotroph cells of   the anterior pituitary where four cleavage sites are used;   adrenocorticotrophin, essential for normal steroidogenesis and the   maintenance of normal adrenal weight, and lipotropin beta are the major end   products. In other tissues, including the hypothalamus, placenta, and   epithelium, all cleavage sites may be used, giving rise to peptides with   roles in pain and energy homeostasis, melanocyte stimulation, and immune   modulation. These include several distinct melanotropins, lipotropins, and   endorphins that are contained within the adrenocorticotrophin and   beta-lipotropin peptides. Mutations in this gene have been associated with   early onset obesity, adrenal insufficiency, and red hair pigmentation.   Alternatively spliced transcript variants encoding the same protein have been   described. [provided by RefSeq, Jul 2008].

其他:

Function:

ACTH stimulates the adrenal glands to release   cortisol.

MSH (melanocyte-stimulating hormone) increases   the pigmentation of skin by increasing melanin production in melanocytes.

Beta-endorphin and Met-enkephalin are endogenous   opiates.

Subcellular   Location:

Secreted.

Tissue   Specificity:

ACTH and MSH are produced by the pituitary gland.

Post-translational   modifications:

Specific enzymatic cleavages at paired basic   residues yield the different active peptides.

O-glycosylated; reducing sugar is probably   N-acetylgalactosamine.

DISEASE:

Defects in POMC may be associated with   susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition   characterized by an increase of body weight beyond the limitation of skeletal   and physical requirements, as the result of excessive accumulation of body   fat.

Defects in POMC are the cause of   pro-opiomelanocortinin deficiency (POMCD) [MIM:609734]. Affected individuals   present early-onset obesity, adrenal insufficiency and red hair.

Similarity:

Belongs to the POMC family.

Gene   ID:

5443

促肾上腺皮质激素(Adrenorticotrophin hormons, ACTH)是垂体前叶细胞分泌的一种多肽激素，是肾上腺皮质活性的主要调节者。抗体可与人的ACTH反应,与多种其它哺乳动物的ACTH有交叉反应，可用于垂体腺瘤的功能性分类，有助于区分原发性和转移型垂体肿瘤，嗜络细胞瘤等部分神经内分泌肿瘤也可出现阳性反应.

Tissue/cell: rat brain   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-ACTH(18-39) Polyclonal Antibody, Unconjugated 1:200,   overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023)   and DAB(C-0010) staining

Tissue/cell: rat brain   tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-ACTH(18-39) Polyclonal Antibody, Unconjugated(1:200,   overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3   conjugated used at 1:200 dilution for 40 minutes at 37°C.   DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei