中/英文名称：

整合素样金属蛋白酶与凝血酶1型抗体/ Anti- ADAMTS1

别名：

A disintegrin and metalloproteinase with   thrombospondin motifs 1; A disintegrin like and metalloprotease with thrombospondin   type 1 motif 1; A disintegrin-like and metalloprotease (reprolysin type) with   thrombospondin type 1 motif 1; ADAM metallopeptidase with thrombospondin type   1 motif 1; ADAM TS 1; ADAM TS1; ADAMTS 1; ADAMTS; C3 C5; KIAA1346; METH 1;   METH1.

产品编号：

zy10019

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   mouse ADAMTS1

交叉反应：

Human, Mouse, Rat, Chicken, Pig, Cow, Horse,   Sheep

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 106kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

ADAMTS1 is a metalloproteinase of the ADAM (A   Disintegrin And Metalloproteinase) family containing disintegrin-like domains.   ADAMTS1, also known as METH1, was first described as a protein elevated in   invasive mouse tumors. Initial findings indicated a role for ADAMTS1 in tumor   progression, since the protein was preferentially expressed in more invasive   tumor cell lines. ADAMTS1 is necessary for normal growth, fertility, and   organ morphology and function.

其他:

Function:

Cleaves aggrecan, a cartilage proteoglycan, and   may be involved in its turnover (By similarity). Has angiogenic inhibitor   activity. Active metalloprotease, which may be associated with various   inflammatory processes as well as development of cancer cachexia. May play a   critical role in follicular rupture.

Subcellular   Location:

Secreted > extracellular space >   extracellular matrix.

Post-translational modifications:

The precursor is cleaved by a furin   endopeptidase.

Similarity:

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 3 TSP type-1 domains.

Gene   ID:

1150459

Antigen: bs-1208P,   0.2ug/100ul

Primary: Antiserum, 1:500,   1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;

Secondary: HRP conjugated   Goat-Anti-Rabbit IgG at 1: 3000;

TMB(C-0024) staining;

Read the data in   MicroplateReader by 450nm.

Tissue/cell: human lung   carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-ADAMTS1 Polyclonal Antibody, Unconjugated 1:200, overnight   at 4°C, followed by conjugation to the secondary antibody and DAB(C-0010)   staining