中/英文名称：

整合素样金属蛋白酶与凝血酶1型-7抗体 (N端抗体)/ Anti- ADAMTS7(NT)

别名：

A disintegrin and metalloproteinase with   thrombospondin motifs 7; ADAM metallopeptidase with thrombospondin type 1   motif 7; A disintegrin and metalloprotease with thrombospondin motifs 7   preproprotein; A disintegrin and metalloproteinase with thrombospondin motifs   7; A disintegrin like and metalloprotease (reprolysin type) with   thrombospondin type 1 motif 7; A disintegrin like and metalloprotease with   thrombospondin type 1 motif 7; ADAM metallopeptidase with thrombospondin type   1 motif 7; ADAM metallopeptidase with thrombospondin type 1 motif 7   preproprotein; ADAM TS 7; ADAM TS7; ADAMTS 7; ADAMTS7; COMPase; DKFZp434H204;   ATS7_MOUSE.

产品编号：

zy10020

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   mouse ADAMTS7

交叉反应：

Human, Mouse, Rat

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 158kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Metalloprotease that may play a role in the   degradation of COMP. Binds 1 zinc ion per subunit. pH dependence: Optimum pH   is between 7.5 and 9.5. [Subcellular location] Secreted, extracellular space,   extracellular matrix (By similarity). Note=Also found associated with the   external cell surface[Tissue specificity] Expressed in heart, brain,   placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in   meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Up-regulated   in articular cartilage and synovium from arthritis patients.

其他:

Function:

Metalloprotease that may play a role in the   degradation of COMP.

Subunit:

Interacts with COMP.

Subcellular   Location:

Secreted, extracellular space, extracellular   matrix. Note=Also found associated with the external cell surface.

Tissue   Specificity:

Expressed in heart, brain, placenta, lung, liver,   skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon,   cartilage, synovium, fat and ligaments.

Post-translational   modifications:

N-glycosylated.

O-glycosylated proteoglycan. Contains chondroitin   sulfate (By similarity).

May be cleaved by a furin endopeptidase (By   similarity). The precursor is sequentially processed.

Similarity:

Contains 1 disintegrin domain.

Contains 1 peptidase M12B domain.

Contains 1 PLAC domain.

Contains 8 TSP type-1 domains.

Gene   ID:

102248157

整合素样金属蛋白酶与凝血酶1型-7用于动脉粥样硬化导致早期血管基质修复作用的研究

Tissue/cell: rat brain   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated 1:200, overnight   at 4°C, followed by conjugation to the secondary antibody and DAB staining

Tissue/cell: rat brain   tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated 1:200, overnight   at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used   at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain   the cell nuclei