中/英文名称：

腺苷受体A3抗体/ Anti-   Adenosine A3 Receptor

别名：

adenosine A3 receptor; A3AR; AD 026; AD026; ADORA   3; ADORA3; bA552M11.5; RP11 552M11.7; TGPCR1; adenosine receptor A3 isoform   1; adenosine receptor A3 isoform 2; AA3R_HUMAN.

产品编号：

zy10030

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ADORA3

交叉反应：

Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Shee

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 35kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications. 
  Optimal working dilutions must be determined by the end user.

背景:

This gene encodes a protein that belongs to the   family of adenosine receptors, which are G-protein-coupled receptors that are   involved in a variety of intracellular signaling pathways and physiological   functions. The receptor encoded by this gene mediates a sustained   cardioprotective function during cardiac ischemia, it is involved in the   inhibition of neutrophil degranulation in neutrophil-mediated tissue injury,   it has been implicated in both neuroprotective and neurodegenerative effects,   and it may also mediate both cell proliferation and cell death. Multiple   transcript variants encoding different isoforms have been found for this   gene. [provided by RefSeq, Jul 2008].

其他:

Function:

Receptor for adenosine. The activity of this   receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible   role in reproduction.

Subcellular   Location:

Cell membrane.

Post-translational   modifications:

Phosphorylation on Ser-317 may be crucial for   rapid desensitization.

Similarity:

Belongs to the G-protein coupled receptor 1   family.

Gene   ID:

140

ADORA-3(腺苷受体A3)属于G蛋白偶联受体家族，在腺苷调节细胞活力和生长中起关键作用，并且在很多肿瘤中表达增高，A3AR可能成为肿瘤诊断标志物和治疗靶点。

Tissue/cell: human gastric   carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min; 
  Incubation: Anti-ADORA3 Polyclonal Antibody, Unconjugated 1:200, overnight at   4°C, followed by conjugation to the secondary antibody and DAB staining

Protein: line1, human colon   carcinoma lysates, 30ug; line2, human gastric carcinoma lysates, 30ug; 
  Primary: Anti-ADORA3 at 1:200; 
  Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000; 
  ECL excitated the fluorescence; 
  Predicted band size : 35kD
  Observed band size : 35kD