中/英文名称：

脂肪组织分化相关蛋白抗体/ Anti- ADFP

别名：

ADFP; Adipophilin; Adipose differentiation   related protein; Adipose differentiation-related protein; Perilipin-2; PLIN2;   PLIN2_HUMAN; adipophilin; Adipose differentiation related protein; ADRP;   MGC10598

产品编号：

zy10023

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ADFP

交叉反应：

Human, Mouse, Rat

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 48kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500    IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

The protein encoded by this gene belongs to the   perilipin family, members of which coat intracellular lipid storage droplets.   This protein is associated with the lipid globule surface membrane material,   and maybe involved in development and maintenance of adipose tissue. However,   it is not restricted to adipocytes as previously thought, but is found in a   wide range of cultured cell lines, including fibroblasts, endothelial and   epithelial cells, and tissues, such as lactating mammary gland, adrenal   cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver   cirrhosis, suggesting that it may serve as a marker of lipid accumulation in   diverse cell types and diseases. Alternatively spliced transcript variants   have been found for this gene. [provided by RefSeq, Mar 2011]

其他:

Function:

May be involved in development and maintenance of   adipose tissue.

Subcellular   Location:

Membrane; Peripheral membrane protein.

Tissue   Specificity:

Milk lipid globules.

Post-translational   modifications:

Acylated; primarily with C14, C16 and C18 fatty   acids.

Similarity:

Belongs to the perilipin family.

Gene   ID:

123

Adipophilin（ADFP）是一种脂滴周围主要的相关蛋白，它大量分布在脂质蓄积正常或不正常的细胞中，是脂质蓄积的一个特异性标记物。它在脂肪细胞分化早期就有很高的表达，但当脂肪细胞成熟后，它的表达就明显减少，故称；脂肪分化相关蛋白。

ADFP在很多组织器官中都发挥重要作用，它不仅参与脂肪细胞的脂质代谢、脂滴的形成及肝内三酰甘油（TO）的合成与代谢，还能促进巨噬细胞、平滑肌细胞的泡沫化，长链脂肪酸的摄取，乳汁的分泌等。

ADFP的表达异常与动脉粥样硬化、胰岛素抵抗和肿瘤等病理过程密切相关。

Tissue/cell: human placenta   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-ADFP Polyclonal Antibody, Unconjugated 1:200, overnight at   4°C, followed by conjugation to the secondary antibody and DAB staining