中/英文名称：

脂联素抗体/ Anti-   Adiponectin

别名：

30 kDa adipocyte complement related protein; 30   kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO_HUMAN;   Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte   complement related 30 kDa protein; Adipocyte complement related protein of 30   kDa; Adipocyte complement-related 30 kDa protein; Adiponectin; AdipoQ;   Adipose most abundant gene transcript 1; Adipose most abundant gene   transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN;   APM 1; apM-1; ApM1; C1q and collagen domain-containing protein; GBP 28;   GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding   protein.

产品编号：

zy10026

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human Adiponectin

交叉反应：

Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 30kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Adiponectin: A protein hormone produced and   secreted exclusively by adipocytes (fat cells) that regulates the metabolism   of lipids and glucose. Adiponectin influences the body's response to insulin.   Adiponectin also has antiinflammatory effects on the cells lining the walls   of blood vessels. High blood levels of adiponectin are associated with a   reduced risk of heart attack. Low levels of adiponectin are found in people   who are obese (and who are at increased risk of a heart attack). Adipocytes   produce and secrete a number of proteins, including leptin, adipsin,   properdin, and tumor necrosis factor (TNF). An adipose tissue-specific factor   was isolated and termed APM1 (AdiPose Most abundant gene transcript 1). The   protein product of APM1 is adiponectin. The APM1 gene maps to chromosome   3q27.

其他:

Function:

Important adipokine involved in the control of   fat metabolism and insulin sensitivity, with direct anti-diabetic,   anti-atherogenic and anti-inflammatory activities. Stimulates AMPK   phosphorylation and activation in the liver and the skeletal muscle,   enhancing glucose utilization and fatty-acid combustion. Antagonizes   TNF-alpha by negatively regulating its expression in various tissues such as   liver and macrophages, and also by counteracting its effects. Inhibits   endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a   role in cell growth, angiogenesis and tissue remodeling by binding and   sequestering various growth factors with distinct binding affinities,   depending on the type of complex, LMW, MMW or HMW.

Subunit:

Homomultimer. Forms trimers, hexamers and 12- to   18-mers. The trimers (low molecular weight complexes / LMW) are assembled via   non-covalent interactions of the collagen-like domains in a triple helix and   hydrophobic interactions within the globular C1q domain. Several trimers can   associate to form disulfide-linked hexamers (middle molecular weight   complexes / MMW) and larger complexes (higher molecular weight / HMW). The   HMW-complex assembly may rely aditionally on lysine hydroxylation and   glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW   complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9A   via the C1q domain (heterotrimeric complex).

Subcellular   Location:

Secreted.

Tissue   Specificity:

Synthesized exclusively by adipocytes and   secreted into plasma.

Post-translational   modifications:

Hydroxylated Lys-33 was not identified in   PubMed:16497731, probably due to poor representation of the N-terminal   peptide in mass fingerprinting.

HMW complexes are more extensively glycosylated   than smaller oligomers. Hydroxylation and glycosylation of the lysine   residues within the collagene-like domain of adiponectin seem to be   critically involved in regulating the formation and/or secretion of HMW complexes   and consequently contribute to the insulin-sensitizing activity of   adiponectin in hepatocytes (By similarity).

O-glycosylated. Not N-glycosylated. O-linked   glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the   oxygen atom of post-translationally added hydroxyl groups. Sialylated to   varying degrees depending on tissue. Thr-22 appears to be the major site of   sialylation. Higher sialylation found in SGBS adipocytes than in HEK   fibroblasts. Sialylation is not required neither for heterodimerization nor   for secretion. Not sialylated on the glycosylated hydroxylysines.   Desialylated forms are rapidly cleared from the circulation.

DISEASE:

Adiponectin deficiency (ADPND) [MIM:612556]: A   condition that results in very low concentrations of plasma adiponectin.   Note=The disease is caused by mutations affecting the gene represented in   this entry.

Diabetes mellitus, non-insulin-dependent (NIDDM)   [MIM:125853]: A multifactorial disorder of glucose homeostasis caused by a   lack of sensitivity to the body's own insulin. Affected individuals usually   have an obese body habitus and manifestations of a metabolic syndrome   characterized by diabetes, insulin resistance, hypertension and   hypertriglyceridemia. The disease results in long-term complications that   affect the eyes, kidneys, nerves, and blood vessels. Note=Disease   susceptibility is associated with variations affecting the gene represented   in this entry.

Similarity:

Contains 1 C1q domain.

Contains 1 collagen-like domain.

Gene   ID:

9370

脂联素又被称作Acrp30、apM1、AdipoQ、GBP28。最初, 脂联素是在人体皮下脂肪组织、血浆和鼠科动物的脂肪细胞中被发现人体内的脂联素由244个氨基酸组成,分子量为27KD。

脂联素影响脂肪和糖类的代谢，还可以降低多种粘附分子(VCAM-1、ICAM-1、选择素-E) 在内皮细胞的表达。并影响着肿瘤坏死因子（TNF）分泌，近年来主要用于糖尿病的研究。

脂联素作为一种胰岛素超敏化激素(An Insulin–sensitizing Hormone),可以增加促进骨骼肌细胞的脂肪酸氧化和糖吸收,明显加强胰岛素的糖元异生作用,抑制肝脏的糖生成,是机体的脂质代谢和血糖稳态的调控网络中的重要调节因子，在脂肪细胞分泌的具有生物活性的一类蛋白质因子中脂联素是脂肪组织基因表达最丰富的蛋白质产物之一,大量存在于血液循环中。在人体内以3-30ug/ml的浓度出现在循环血浆中。

Tissue/cell: human colon   carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated 1:200,   overnight at 4°C, followed by conjugation to the secondary antibody and DAB   staining

Tissue/cell: mouse liver   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-Adiponectin Polyclonal Antibody, Unconjugated 1:200,   overnight at 4°C, followed by conjugation to the secondary antibody and DAB   staining

Protein: line1, rat liver   lysates, 30ug; line2, mouse intestine lysates, 30ug;
  Primary: Anti-Adiponectin at 1:200;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG  at 1: 3000;
  ECL excitated the fluorescence;
  Predicted band size : 25kD
  Observed band size : 25kD