中/英文名称：

脂联素受体1抗体/ Anti- Adiponectin Receptor 1

别名：

ACDCR1; ADIPO R1; Adiponectin receptor protein 1;   ADIPOR 1; ADIPOR1; CGI-45; CGI-45 protein;FLJ25385; PAQR1; Progestin and   adipoQ receptor family member I; TESBP1A; ADR1_HUMAN.

产品编号：

zy10024

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human Adiponectin Receptor 1 (241-270aa)

交叉反应：

Human, Mouse, Rat, Dog, Rabbit

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 42kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Acrp30 is a hormone secreted by adipocytes that   acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin   in the blood are decreased under conditions of obesity, insulin resistance   and type 2 diabetes. Administration of adiponectin causes glucose-lowering   effects and ameliorates insulin resistance in mice. Conversely,   adiponectin-deficient mice exhibit insulin resistance and diabetes. This   insulin-sensitizing effect of adiponectin seems to be mediated by an increase   in fatty-acid oxidation through activation of AMP kinase and PPAR- . Cloning   of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and   AdipoR2) have shown that AdipoR1 is abundantly expressed in skeletal muscle,   whereas AdipoR2 is predominantly expressed in the liver.

其他:

Function:

Receptor for globular and full-length adiponectin   (APM1), an essential hormone secreted by adipocytes that acts as an   antidiabetic. Probably involved in metabolic pathways that regulate lipid   metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA   ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has   some high-affinity receptor for globular adiponectin but low-affinity   receptor for full-length adiponectin.

Subunit:

May form homo and heteromultimers.

Subcellular   Location:

Membrane; Multi-pass membrane protein.   Note=Localized to the cell membrane and intracellular organelles.

Tissue   Specificity:

Widely expressed. Highly expressed in skeletal   muscle. Expressed at intermediate level in brain, heart, spleen, kidney,   liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in   colon, thymus and small intestine.

Similarity:

Belongs to the ADIPOR family.

Gene   ID:51094

脂联素与受体结合后具有增强胰岛素敏感性，抗高血糖，抗动脉粥样硬化等生物学效应，任何增加或减少脂联素及其受体表达的方法都影响这些疾病的发生和发展。脂联素受体-1(AdipoR1和AdipoR2)同属于PAQR家族，具有七次跨膜蛋白的特有结构。

Tissue/cell: human rectal   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated   1:300, overnight at 4°C, followed by conjugation to the secondary antibody   and DAB staining

Tissue/cell: rat pancreas   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-Adiponectin Receptor 1 Polyclonal Antibody, Unconjugated   1:300, overnight at 4°C, followed by conjugation to the secondary antibody   and DAB staining