中/英文名称：

肾上腺髓质素抗体/ Anti-ADM

别名：

Adrenomedullin; ADM; ADM precursor; ADML_HUMAN.

产品编号：

zy10027

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ADM N-terminus

交叉反应：

Human, Mouse, Rat, Dog, Cow, Horse

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 20kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Adrenomedullin, a hypotensive peptide found in   human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular   disulfide bond, and shows a slight homology with the calcitonin gene-related   peptide. It may function as a hormone in circulation control because it is   found in blood in a considerable concentration. The precursor, called   preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human   adrenomedullin mRNA was found to be highly expressed in several tissues.   Genomic ADM DNA consists of4 exons and 3 introns, with the 5-prime flanking   region containing TATA, CAAT, and GC boxes. There are also multiple binding   sites for activator protein-2 and a cAMP-regulated enhancer element.

其他:

Function:

AM and PAMP are potent hypotensive and   vasodilatator agents. Numerous actions have been reported most related to the   physiologic control of fluid and electrolyte homeostasis. In the kidney, am   is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion   by direct adrenal actions. In pituitary gland, both peptides at   physiologically relevant doses inhibit basal ACTH secretion. Both peptides   appear to act in brain and pituitary gland to facilitate the loss of plasma   volume, actions which complement their hypotensive effects in blood vessels.

Subcellular   Location:

Secreted.

Tissue   Specificity:

Highest levels found in pheochromocytoma and   adrenal medulla. Also found in lung, ventricle and kidney tissues.

Similarity:

Belongs to the adrenomedullin family.

Gene   ID:

133

肾上腺髓质素（Adrenomedullin， AM）是广泛分布在肾上腺髓质、肺、心、肝、肾等器官。

Tissue/cell: rat brain   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-ADM/AM/Adrenomedullin Polyclonal Antibody, Unconjugated 1:200,   overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining