中/英文名称：

凋亡抑制因子5抗体/ Anti-API5

别名：

apoptosis inhibitor 5; apoptosis inhibitory   protein 5; API5; AAC-11; AAC11; API5L1; AAC 11; Antiapoptosis clone 11   protein; API5 like 1; Cell migration inducing gene 8 protein; Fibroblast   growth factor 2 interacting factor 2; FIF; Migration inducing protein MIG8;   MIG 8; MIG8; Protein XAGL; API5_HUMAN.

产品编号：

zy10001

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human API5

交叉反应：

Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea   Pig

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 57kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500    
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Apoptosis inhibitor 5 (API5) is an antiapoptotic   factor which may have a role in protein assembly. API5 expression is often   upregulated in tumor cells, particularly in metastatic cells. It is a   critical determinant of E2F1-induced apoptosis, acting downstream of E2F to   suppress E2F-dependent apoptosis without generally blocking E2F-dependent   transcription. Alternate Names: AAC 11; AAC11; Antiapoptosis clone 11   protein; API 5; API5; API5 like 1; API5L1; Cell migration inducing gene 8   protein; Fibroblast growth factor 2 interacting factor 2; FIF; MIG 8; MIG8;   Migration inducing protein MIG8; Protein XAGL.

其他:

Function:

Antiapoptotic factor that may have a role in   protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it   suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation.   Also known to efficiently suppress E2F1-induced apoptosis. Its depletion   enhances the cytotoxic action of the chemotherapeutic drugs.

Subunit:

Monomer. Interacts with FGF2 and ACIN1.

Subcellular Location:

Nucleus. Cytoplasm. Note=Mainly nuclear. Can also   be cytoplasmic.

Isoform 3: Cytoplasm.

Tissue   Specificity:

Expressed in all tissues tested, including heart,   brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest   levels in heart, pancreas and placenta. Highly expressed in several cancers.   Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in   non-small cell lung cancer.

Post-translational   modifications:

Acetylation at Lys-251 impairs antiapoptotic   function.

Similarity:

Belongs to the API5 family.

Gene   ID: 8539

API-5是细胞凋亡的一种抑制因子、参与细胞凋亡调控，可以用于各种恶性肿瘤的细胞凋亡的研究.

Antigen: 0.2ug/100ul

Primary: Antiserum, 1:500,   1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;

Secondary: HRP conjugated   Goat Anti-Rabbit IgGat 1: 5000;

TMB staining;

Read the data in   MicroplateReader by 450nm.

Protein: mouse heart   lysates(A); mouse pancreas lysates(B);
  Primary: Anti-API5 at 1:300;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
  ECL excitated the fluorescence;
  Predicted band size : 57 kD
  Observed band size :57 kD