中/英文名称：

纤维状肌动蛋白抗体/ Anti-F-Actin

别名：

f actin; Filamentous actin; F-actin capping   protein alpha subunit; CapZ alpha-1; CAZA1_HUMAN; CAPZA1.

产品编号：

zy10038

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human F-actin capping protein alpha subunit

交叉反应：

Human, Mouse, Rat, Pig, Cow, Sheep

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 42kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Actins are highly conserved proteins that are   involved in various types of cell motility and are ubiquitously expressed in   all eukaryotic cells .G-Actin (globular actin) with bound ATP can polymerise   to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis   of the filament similar to a double helix. F-Actin may also undergo a process   called treadmilling, in which filament length remains constant and actin   monomers add at one end and dissociated at the other.

其他:

Function:

Alpha-2 adrenergic receptors mediate the   catecholamine-induced inhibition of adenylate cyclase through the action of G   proteins. The rank order of potency for agonists of this receptor is   oxymetazoline > clonidine > epinephrine > norepinephrine >   phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin   = p-octopamine. For antagonists, the rank order is yohimbine >   phentolamine = mianserine > chlorpromazine = spiperone = prazosin >   propanolol > alprenolol = pindolol.

Subcellular   Location:

Cell membrane.

Similarity:

Belongs to the G-protein coupled receptor 1   family. Adrenergic receptor subfamily.

ADRA2A sub-subfamily.

Gene   ID:

150

通道蛋白（Channel Protein）AQP 3主要分布于肾脏集合管上皮细胞基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.有学者认为：AQP-3的阳性率与某些肿瘤分化程度有关（胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等）

Antigen: 0.2ug/100ul

Primary: Antiserum, 1:500,   1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;

Secondary: HRP conjugated   Goat-Anti-Rabbit IgG at 1: 5000;

TMB(C-0024) staining;

Read the data in   MicroplateReader by 450nm.

Tissue/cell: rat taste buds;   4% Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate   buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous   peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat   serum) at 37℃ for 20 min;

Incubation: Anti-AQP-3   Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by   conjugation to the secondary antibody and DAB staining

Tissue/cell: rat tongue tissue; 4%   Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate buffer ( 0.01M, pH   6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen   peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20   min;

Incubation: Anti-AQP-3 Polyclonal Antibody,   Unconjugated 1:200, overnight at 4°C, followed by conjugation to the   secondary antibody and DAB staining